Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins w...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Schmitz, Jonathan E., Daniel, Anu, Collin, Mattias, Schuch, Raymond, Fischetti, Vincent A.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: American Society for Microbiology (ASM) 2008
Gaiak:
Methods
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2258639/
https://ncbi.nlm.nih.gov/pubmed/18083885
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01864-07
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