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Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening
A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins w...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2258639/ https://ncbi.nlm.nih.gov/pubmed/18083885 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01864-07 |
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