Rapid DNA Library Construction for Functional Genomic and Metagenomic Screening

A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins w...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Main Authors: Schmitz, Jonathan E., Daniel, Anu, Collin, Mattias, Schuch, Raymond, Fischetti, Vincent A.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2008
Teme:
Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2258639/
https://ncbi.nlm.nih.gov/pubmed/18083885
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01864-07
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki