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Fluorescent Methods to Study Transcription Initiation and Transition into Elongation

The DNA-dependent RNA polymerases induce specific conformational changes in the promoter DNA during transcription initiation. Fluorescence spectroscopy sensitively monitors these DNA conformational changes in real time and at equilibrium providing powerful ways to estimate interactions in transcript...

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Bibliographic Details
Published in:EXS
Main Authors: Deshpande, Aishwarya P., Sultana, Shemaila, Patel, Smita S.
Format: Artigo
Language:Inglês
Published: 2014
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4430081/
https://ncbi.nlm.nih.gov/pubmed/25095993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-3-0348-0856-9_6
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