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One-Pot Production of l-threo-3-Hydroxyaspartic Acid Using Asparaginase-Deficient Escherichia coli Expressing Asparagine Hydroxylase of Streptomyces coelicolor A3(2)

We developed a novel process for efficient synthesis of l-threo-3-hydroxyaspartic acid (l-THA) using microbial hydroxylase and hydrolase. A well-characterized mutant of asparagine hydroxylase (AsnO-D241N) and its homologous enzyme (SCO2693-D246N) were adaptable to the direct hydroxylation of l-aspar...

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Publicat a:Appl Environ Microbiol
Autors principals: Hara, Ryotaro, Nakano, Masashi, Kino, Kuniki
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2015
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4421054/
https://ncbi.nlm.nih.gov/pubmed/25795668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03963-14
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