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Highly efficient CRISPR/Cas9-mediated TAR cloning of genes and chromosomal loci from complex genomes in yeast

Transformation-associated recombination (TAR) protocol allowing the selective isolation of full-length genes complete with their distal enhancer regions and entire genomic loci with sizes up to 250 kb from complex genomes in yeast S. cerevisiae has been developed more than a decade ago. However, its...

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Библиографические подробности
Опубликовано в: :Nucleic Acids Res
Главные авторы: Lee, Nicholas C.O., Larionov, Vladimir, Kouprina, Natalay
Формат: Artigo
Язык:Inglês
Опубликовано: Oxford University Press 2015
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4417148/
https://ncbi.nlm.nih.gov/pubmed/25690893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv112
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