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Optimal excitation and emission wavelengths to analyze amino acids and optimize neurotransmitters quantification using precolumn OPA-derivatization by HPLC
We describe an analytical methodology to obtain high sensitivity and better resolution through the study of fluorometric excitation (λ (ex)) and emission (λ (em)) spectrum wavelengths of OPA–amino acids. The spectrum emission study revealed a maximum signal peak at 450 nm for aspartate and glutamine...
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| Veröffentlicht in: | Amino Acids |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Springer Vienna
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4412611/ https://ncbi.nlm.nih.gov/pubmed/25691143 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00726-015-1925-1 |
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