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Optimal excitation and emission wavelengths to analyze amino acids and optimize neurotransmitters quantification using precolumn OPA-derivatization by HPLC

We describe an analytical methodology to obtain high sensitivity and better resolution through the study of fluorometric excitation (λ (ex)) and emission (λ (em)) spectrum wavelengths of OPA–amino acids. The spectrum emission study revealed a maximum signal peak at 450 nm for aspartate and glutamine...

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Bibliografske podrobnosti
izdano v:Amino Acids
Main Authors: Perucho, J., Gonzalo-Gobernado, R., Bazan, E., Casarejos, M. J., Jiménez-Escrig, A., Asensio, M. J., Herranz, A. S.
Format: Artigo
Jezik:Inglês
Izdano: Springer Vienna 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4412611/
https://ncbi.nlm.nih.gov/pubmed/25691143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00726-015-1925-1
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