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A broad pH range indicator-based spectrophotometric assay for true lipases using tributyrin and tricaprylin
A continuous assay is proposed for the screening of acidic, neutral, or alkaline lipases using microtiter plates, emulsified short- and medium-chain TGs, and a pH indicator. The lipase activity measurement is based on the decrease of the pH indicator optical density due to protonation which is cause...
Gespeichert in:
| Veröffentlicht in: | J Lipid Res |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
The American Society for Biochemistry and Molecular Biology
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4409282/ https://ncbi.nlm.nih.gov/pubmed/25748441 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D052837 |
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