An ultraviolet spectrophotometric assay for the screening of sn-2-specific lipases using 1,3-O-dioleoyl-2-O-α-eleostearoyl-sn-glycerol as substrate

In the present study, we propose a continuous assay for the screening of sn-2 lipases by using triacylglycerols (TAGs) from Aleurites fordii seed (tung oil) and a synthetic TAG containing the α-eleostearic acid at the sn-2 position and the oleic acid (OA) at the sn-1 and sn-3 positions [1,3-O-dioleo...

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Detalhes bibliográficos
Main Authors: Mendoza, Lilia D., Rodriguez, Jorge A., Leclaire, Julien, Buono, Gerard, Fotiadu, Frédéric, Carrière, Frédéric, Abousalham, Abdelkarim
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2012
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3243475/
https://ncbi.nlm.nih.gov/pubmed/22114038
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D019489
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