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GENOME EDITING IN HUMAN STEM CELLS

The use of custom-engineered sequence-specific nucleases (including CRISPR/Cas9, ZFN, and TALEN) allows genetic changes in human cells to be easily made with much greater efficiency and precision than before. Engineered double-stranded DNA breaks can efficiently disrupt genes, or, with the right don...

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Detalhes bibliográficos
Publicado no:Methods Enzymol
Main Authors: Byrne, Susan M., Mali, Prashant, Church, George M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4408990/
https://ncbi.nlm.nih.gov/pubmed/25398338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-801185-0.00006-4
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