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Molecular cloning and characterization of the cDNA coding for the biotin-containing subunit of 3-methylcrotonoyl-CoA carboxylase: identification of the biotin carboxylase and biotin-carrier domains.

Soybean genomic clones were isolated based on hybridization to probes that code for the conserved biotinylation domain of biotin-containing enzymes. The corresponding cDNA was isolated and expressed in Escherichia coli through fusion to the bacterial trpE gene. The resulting chimeric protein was bio...

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Autors principals: Song, J, Wurtele, E S, Nikolau, B J
Format: Artigo
Idioma:Inglês
Publicat: 1994
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC44080/
https://ncbi.nlm.nih.gov/pubmed/8016064
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