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Improved Procedure for the Estimation of Nanogram Quantities of Indole-3-acetic Acid in Plant Extracts using the Indolo-α-pyrone Fluorescence Method

The indolo-α-pyrone fluorescence method of determining indole-3-acetic acid (IAA) is improved by adding butylated hydroxytoluene (BHT), an antioxidant, to samples: addition of BHT increases the fluorescence intensities and decreases their variability so that amounts of IAA as small as 0.1 to 1 nanog...

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Bibliographic Details
Published in:Plant Physiol
Main Authors: Iino, Moritoshi, Yu, Richard S.-T., Carr, Denis J.
Format: Artigo
Language:Inglês
Published: Oxford University Press 1980
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440797/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661584/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.66.6.1099
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