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Method to Obtain a Chlorophyll-free Preparation of Intact Mitochondria from Spinach Leaves

Mitochondria from green leaves of spinach have been prepared using a three-step procedure involving differential centrifugation, partition in an aqueous dextran polyethylene glycol two-phase system and Percoll gradient centrifugation. The mitochondrial fractions after the different steps of purifica...

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Detalhes bibliográficos
Main Authors: Bergman, Anders, Gardeström, Per, Ericson, Ingemar
Formato: Artigo
Idioma:Inglês
Publicado em: 1980
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC440650/
https://ncbi.nlm.nih.gov/pubmed/16661452
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