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Enzymic Determination of Metabolites in the Subcellular Compartments of Spinach Protoplasts

A method for determining the subcellular metabolite levels in spinach protoplasts is described. The protoplasts are disrupted by centrifugation through a nylon net, releasing intact chloroplasts which pass through a layer of silicone oil into perchloric acid while the remaining cytoplasmic component...

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Bibliografske podrobnosti
izdano v:Plant Physiol
Principais autores: Wirtz, Wolfgang, Stitt, Mark, Heldt, Hans W.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1980
Teme:
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440555/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661385/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.66.1.187
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