Enzymic Determination of Metabolites in the Subcellular Compartments of Spinach Protoplasts
A method for determining the subcellular metabolite levels in spinach protoplasts is described. The protoplasts are disrupted by centrifugation through a nylon net, releasing intact chloroplasts which pass through a layer of silicone oil into perchloric acid while the remaining cytoplasmic component...
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| Publicado no: | Plant Physiol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440555/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661385/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.66.1.187 |
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