Conceptual Error in Determination of NAD(+)-Malic Enzyme in Extracts Containing NAD(+)-Malic Dehydrogenase
The typical procedure for determining NAD(+)-malic enzyme (EC 1.1.1.39) is to calculate the enzyme rate to be ΔA(340)/Δ time after the endogenous NAD(+)-malic dehydrogenase (EC 1.1.1.37) catalyzed reaction has reached equilibrium. This ignores the equilibrium shift of oxaloacetic acid and NADH durin...
Shranjeno v:
| izdano v: | Plant Physiol |
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| Principais autores: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1980
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440497/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661347/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.65.6.1136 |
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