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Conceptual Error in Determination of NAD(+)-Malic Enzyme in Extracts Containing NAD(+)-Malic Dehydrogenase
The typical procedure for determining NAD(+)-malic enzyme (EC 1.1.1.39) is to calculate the enzyme rate to be ΔA(340)/Δ time after the endogenous NAD(+)-malic dehydrogenase (EC 1.1.1.37) catalyzed reaction has reached equilibrium. This ignores the equilibrium shift of oxaloacetic acid and NADH durin...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC440497/ https://ncbi.nlm.nih.gov/pubmed/16661347 |
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