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Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature
Animal cells and cell lines, such as HEK-293 cells, are commonly cultured at 37°C. These cells are often used to express recombinant proteins. Having a higher expression level or a higher protein yield is generally desirable. As we demonstrate in this study, dropping culture temperature to 33°C, but...
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| Publicado no: | PLoS One |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4404257/ https://ncbi.nlm.nih.gov/pubmed/25893827 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0123562 |
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