An Optimized Trichloroacetic Acid/Acetone Precipitation Method for Two-Dimensional Gel Electrophoresis Analysis of Qinchuan Cattle Longissimus Dorsi Muscle Containing High Proportion of Marbling

Longissimus dorsi muscle (LD) proteomics provides a novel opportunity to reveal the molecular mechanism behind intramuscular fat deposition. Unfortunately, the vast amounts of lipids and nucleic acids in this tissue hampered LD proteomics analysis. Trichloroacetic acid (TCA)/acetone precipitation is...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Hao, Ruijie, Adoligbe, Camus, Jiang, Bijie, Zhao, Xianlin, Gui, Linsheng, Qu, Kaixing, Wu, Sen, Zan, Linsen
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4404140/
https://ncbi.nlm.nih.gov/pubmed/25893432
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0124723
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