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One-pot refolding of core histones from bacterial inclusion bodies allows rapid reconstitution of histone octamer
We report an optimized method to purify and reconstitute histone octamer, which utilizes high expression of histones in inclusion bodies but eliminates the time consuming steps of individual histone purification. In the newly modified protocol, Xenopus laevis H2A, H2B, H3, and H4 are expressed indiv...
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| Publicado no: | Protein Expr Purif |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4403241/ https://ncbi.nlm.nih.gov/pubmed/25687285 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2015.02.007 |
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