A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications
RNA research and therapy relies primarily on synthetic RNAs. We employed recombinant RNA technology toward large-scale production of pre-miRNA agents in bacteria, but found the majority of target RNAs were not or negligibly expressed. We thus developed a novel strategy to achieve consistent high-yie...
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| Publicado no: | Nucleic Acids Res |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4402540/ https://ncbi.nlm.nih.gov/pubmed/25800741 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv228 |
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