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Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys(2)-His(2) Zinc-finger Domains

Due to their modularity and ability to be reprogrammed to recognize a wide range of DNA sequences, Cys(2)-His(2) zinc-finger DNA-binding domains have emerged as useful tools for targeted genome engineering. Like many other DNA-binding proteins, zinc-fingers also possess the innate ability to cross c...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Gaj, Thomas, Liu, Jia
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4401377/
https://ncbi.nlm.nih.gov/pubmed/25866937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52814
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