CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently been developed. However, especially when multiple genetic manipulations are required, strain construction is still a time-consuming process. This study describes new CRISPR/Cas9-based approaches for easy, fast s...

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Veröffentlicht in:FEMS Yeast Res
Hauptverfasser: Mans, Robert, van Rossum, Harmen M., Wijsman, Melanie, Backx, Antoon, Kuijpers, Niels G.A., van den Broek, Marcel, Daran-Lapujade, Pascale, Pronk, Jack T., van Maris, Antonius J.A., Daran, Jean-Marc G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2015
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4399441/
https://ncbi.nlm.nih.gov/pubmed/25743786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/femsyr/fov004
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