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Analysis of the Heavy Chain Modification and TSG-6 Activity in Pathological Hyaluronan Matrices

During inflammation and developmental processes, heavy chains (HCs) from inter-α-inhibitor (IαI) are covalently transferred to hyaluronan (HA) via the enzyme tumor-necrosis-factor-stimulated-gene 6 (TSG- 6) to form a HC-HA complex. In this manuscript, we describe a gel-based assay to detect HC-HA an...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Lauer, Mark E., Loftis, Jacqueline, de la Motte, Carol, Hascall, Vincent C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4397960/
https://ncbi.nlm.nih.gov/pubmed/25325979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1714-3_42
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