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Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
mRNA steady state levels vary depending on environmental conditions. Regulation of the steady state accumulation levels of an mRNA ensures that the correct amount of protein is synthesized for the cell’s specific growth conditions. One approach for measuring mRNA decay rates is inhibiting transcript...
Gardado en:
| Publicado en: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2014
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4396921/ https://ncbi.nlm.nih.gov/pubmed/25549102 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52240 |
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