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Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

mRNA steady state levels vary depending on environmental conditions. Regulation of the steady state accumulation levels of an mRNA ensures that the correct amount of protein is synthesized for the cell’s specific growth conditions. One approach for measuring mRNA decay rates is inhibiting transcript...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Peccarelli, Megan, Kebaara, Bessie W.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4396921/
https://ncbi.nlm.nih.gov/pubmed/25549102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52240
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