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Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
mRNA steady state levels vary depending on environmental conditions. Regulation of the steady state accumulation levels of an mRNA ensures that the correct amount of protein is synthesized for the cell’s specific growth conditions. One approach for measuring mRNA decay rates is inhibiting transcript...
Αποθηκεύτηκε σε:
| Τόπος έκδοσης: | J Vis Exp |
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| Κύριοι συγγραφείς: | , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
MyJove Corporation
2014
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4396921/ https://ncbi.nlm.nih.gov/pubmed/25549102 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52240 |
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