Pulse-shaping based two-photon FRET stoichiometry

Förster Resonance Energy Transfer (FRET) based measurements that calculate the stoichiometry of intermolecular interactions in living cells have recently been demonstrated, where the technique utilizes selective one-photon excitation of donor and acceptor fluorophores to isolate the pure FRET signal...

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Bibliographic Details
Published in:Opt Express
Main Authors: Flynn, Daniel C., Bhagwat, Amar R., Brenner, Meredith H., Núñez, Marcos F., Mork, Briana E., Cai, Dawen, Swanson, Joel A., Ogilvie, Jennifer P.
Format: Artigo
Language:Inglês
Published: Optical Society of America 2015
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4394757/
https://ncbi.nlm.nih.gov/pubmed/25836193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.23.003353
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