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Two-photon imaging of multiple fluorescent proteins by phase-shaping and linear unmixing with a single broadband laser
Imaging multiple fluorescent proteins (FPs) by two-photon microscopy has numerous applications for studying biological processes in thick and live samples. Here we demonstrate a setup utilizing a single broadband laser and a phase-only pulse-shaper to achieve imaging of three FPs (mAmetrine, TagRFPt...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Optical Society of America
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3724397/ https://ncbi.nlm.nih.gov/pubmed/23938572 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.21.017256 |
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