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Single genome amplification and standard bulk PCR yield HIV-1 envelope products with similar genotypic and phenotypic characteristics

Recent studies suggest that single genome amplification (SGA) as compared to standard bulk PCR and virus stocks from 293T transfection versus short term passage in peripheral blood mononuclear cells (PBMC) yield a less biased representation of HIV-1 envelope characteristics. In 9 different subjects,...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Etemad, Behzad, Smith, Melissa Ghulam, Gonzalez, Oscar, White, Laura F, Sagar, Manish
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4394631/
https://ncbi.nlm.nih.gov/pubmed/25681527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2015.01.006
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