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Single-molecule site-specific detection of protein phosphorylation with a nanopore
We demonstrate single-molecule site-specific detection of protein phosphorylation with protein nanopore technology. A model protein, thioredoxin, was phosphorylated at two adjacent sites. Analysis of the ionic current amplitude and noise, as the protein unfolds and moves through an α-hemolysin pore,...
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| Publicado no: | Nat Biotechnol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4391620/ https://ncbi.nlm.nih.gov/pubmed/24441471 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2799 |
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