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Single-molecule site-specific detection of protein phosphorylation with a nanopore

We demonstrate single-molecule site-specific detection of protein phosphorylation with protein nanopore technology. A model protein, thioredoxin, was phosphorylated at two adjacent sites. Analysis of the ionic current amplitude and noise, as the protein unfolds and moves through an α-hemolysin pore,...

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Vydáno v:Nat Biotechnol
Hlavní autoři: Rosen, Christian B., Rodriguez-Larrea, David, Bayley, Hagan
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4391620/
https://ncbi.nlm.nih.gov/pubmed/24441471
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2799
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