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Identification of novel esterase-active enzymes from hot environments by use of the host bacterium Thermus thermophilus

Functional metagenomic screening strategies, which are independent of known sequence information, can lead to the identification of truly novel genes and enzymes. Since E. coli has been used exhaustively for this purpose as a host, it is important to establish alternative expression hosts and to use...

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Bibliographische Detailangaben
Veröffentlicht in:Front Microbiol
Hauptverfasser: Leis, Benedikt, Angelov, Angel, Mientus, Markus, Li, Haijuan, Pham, Vu T. T., Lauinger, Benjamin, Bongen, Patrick, Pietruszka, Jörg, Gonçalves, Luís G., Santos, Helena, Liebl, Wolfgang
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4389547/
https://ncbi.nlm.nih.gov/pubmed/25904908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2015.00275
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