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Establishment of a Counter-selectable Markerless Mutagenesis System in Veillonella atypica

Using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the Veillonella genus. This system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in Veillonella.

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Bibliografske podrobnosti
izdano v:J Microbiol Methods
Main Authors: Zhou, Peng, Li, Xiaoli, Qi, Fengxia
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4388892/
https://ncbi.nlm.nih.gov/pubmed/25771833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2015.03.010
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