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Establishment of a Counter-selectable Markerless Mutagenesis System in Veillonella atypica

Using an alternative sigma factor ecf3 as target, we successfully established the first markerless mutagenesis system in the Veillonella genus. This system will be a valuable tool for mutagenesis of multiple genes for gene function analysis as well as for gene regulation studies in Veillonella.

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Detalhes bibliográficos
Publicado no:J Microbiol Methods
Main Authors: Zhou, Peng, Li, Xiaoli, Qi, Fengxia
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4388892/
https://ncbi.nlm.nih.gov/pubmed/25771833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2015.03.010
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