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Fine-tuning Interaction between Aminoacyl-tRNA Synthetase and tRNA for Efficient Synthesis of Proteins Containing Unnatural Amino Acids
[Image: see text] By using a directed evolution approach, we have identified aminoacyl-tRNA synthetase variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to the amber nonsense codon in bacteria. We demonstrated that the optimization...
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| Udgivet i: | ACS Synth Biol |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Chemical
Society
2014
|
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4384836/ https://ncbi.nlm.nih.gov/pubmed/24847685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb500195w |
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