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Fine-tuning Interaction between Aminoacyl-tRNA Synthetase and tRNA for Efficient Synthesis of Proteins Containing Unnatural Amino Acids

[Image: see text] By using a directed evolution approach, we have identified aminoacyl-tRNA synthetase variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to the amber nonsense codon in bacteria. We demonstrated that the optimization...

Πλήρης περιγραφή

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Τόπος έκδοσης:ACS Synth Biol
Κύριοι συγγραφείς: Wang, Nanxi, Ju, Tong, Niu, Wei, Guo, Jiantao
Μορφή: Artigo
Γλώσσα:Inglês
Έκδοση: American Chemical Society 2014
Διαθέσιμο Online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4384836/
https://ncbi.nlm.nih.gov/pubmed/24847685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/sb500195w
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