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Proximity biotinylation and affinity purification are complementary approaches for the interactome mapping of chromatin-associated protein complexes
Mapping protein-protein interactions for chromatin-associated proteins remains challenging. Here we explore the use of BioID, a proximity biotinylation approach in which a mutated biotin ligase (BirA*) is fused to a bait of interest, allowing for the local activation of biotin and subsequent biotiny...
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| Publicado no: | J Proteomics |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4383713/ https://ncbi.nlm.nih.gov/pubmed/25281560 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2014.09.011 |
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