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Sensitivity and specificity of immunoprecipitation of DNA containing 5-Methylcytosine

BACKGROUND: Attempts to enrich or identify DNA with cytosine methylation have been commonly carried out using anti-5-methylcytosine or anti-MBD2 (methyl-CpG binding domain protein 2) antibody in immunoprecipitation (IP) assays. However, a careful and systematic control experiment to examine the sens...

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Detalhes bibliográficos
Publicado no:BMC Res Notes
Main Authors: Okitsu, Cindy Y, Hsieh, Chih-Lin
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4379710/
https://ncbi.nlm.nih.gov/pubmed/25888776
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-015-1069-0
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