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Early Development of Cloned Bovine Embryos Produced from Oocytes Enucleated by Fluorescence Metaphase II Imaging Using a Conventional Halogen-Lamp Microscope

Enucleation of a recipient oocyte is one of the key processes in the procedure of somatic cell nuclear transfer (SCNT). However, especially in bovine species, lipid droplets spreading in the ooplasm hamper identification and enucleation of metaphase II (MII) chromosomes, and thereby the success rate...

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Bibliografski detalji
Izdano u:Cell Reprogram
Glavni autori: Iwamoto, Daisaku, Yamagata, Kazuo, Kishi, Masao, Hayashi-Takanaka, Yoko, Kimura, Hiroshi, Wakayama, Teruhiko, Saeki, Kazuhiro
Format: Artigo
Jezik:Inglês
Izdano: Mary Ann Liebert, Inc. 2015
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Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4378878/
https://ncbi.nlm.nih.gov/pubmed/25826723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/cell.2014.0086
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