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Early Development of Cloned Bovine Embryos Produced from Oocytes Enucleated by Fluorescence Metaphase II Imaging Using a Conventional Halogen-Lamp Microscope

Enucleation of a recipient oocyte is one of the key processes in the procedure of somatic cell nuclear transfer (SCNT). However, especially in bovine species, lipid droplets spreading in the ooplasm hamper identification and enucleation of metaphase II (MII) chromosomes, and thereby the success rate...

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Publicado en:Cell Reprogram
Autores principales: Iwamoto, Daisaku, Yamagata, Kazuo, Kishi, Masao, Hayashi-Takanaka, Yoko, Kimura, Hiroshi, Wakayama, Teruhiko, Saeki, Kazuhiro
Formato: Artigo
Lenguaje:Inglês
Publicado: Mary Ann Liebert, Inc. 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4378878/
https://ncbi.nlm.nih.gov/pubmed/25826723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/cell.2014.0086
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