Early Development of Cloned Bovine Embryos Produced from Oocytes Enucleated by Fluorescence Metaphase II Imaging Using a Conventional Halogen-Lamp Microscope

Enucleation of a recipient oocyte is one of the key processes in the procedure of somatic cell nuclear transfer (SCNT). However, especially in bovine species, lipid droplets spreading in the ooplasm hamper identification and enucleation of metaphase II (MII) chromosomes, and thereby the success rate...

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Vydáno v:Cell Reprogram
Hlavní autoři: Iwamoto, Daisaku, Yamagata, Kazuo, Kishi, Masao, Hayashi-Takanaka, Yoko, Kimura, Hiroshi, Wakayama, Teruhiko, Saeki, Kazuhiro
Médium: Artigo
Jazyk:Inglês
Vydáno: Mary Ann Liebert, Inc. 2015
Témata:
Original Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4378878/
https://ncbi.nlm.nih.gov/pubmed/25826723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/cell.2014.0086
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