A Method to Quantify FRET Stoichiometry with Phasor Plot Analysis and Acceptor Lifetime Ingrowth

FRET is widely used for the study of protein-protein interactions in biological samples. However, it is difficult to quantify both the FRET efficiency (E) and the affinity (K(d)) of the molecular interaction from intermolecular FRET signals in samples of unknown stoichiometry. Here, we present a met...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Chen, WeiYue, Avezov, Edward, Schlachter, Simon C., Gielen, Fabrice, Laine, Romain F., Harding, Heather P., Hollfelder, Florian, Ron, David, Kaminski, Clemens F.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2015
Assuntos:
Biophysical Letter
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4375440/
https://ncbi.nlm.nih.gov/pubmed/25762312
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.01.012
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