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Cell-free eukaryotic systems for the production, engineering, and modification of scFv antibody fragments

Open cell-free translation systems based on Escherichia coli cell lysates have successfully been used to produce antibodies and antibody fragments. In this study, we demonstrate the cell-free expression of functional single-chain antibody variable fragments (scFvs) in a eukaryotic and endotoxin-free...

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Bibliographic Details
Published in:Eng Life Sci
Main Authors: Stech, Marlitt, Hust, Michael, Schulze, Corina, Dübel, Stefan, Kubick, Stefan
Format: Artigo
Language:Inglês
Published: BlackWell Publishing Ltd 2014
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4374706/
https://ncbi.nlm.nih.gov/pubmed/25821419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.201400036
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