Stem-loop IV of 5S rRNA lies close to the peptidyltransferase center.

A DNA fragment containing the Escherichia coli 5S rDNA sequence linked to a T7 promoter was prepared by PCR from an M13 clone carrying the 5S-complementary sequence. The DNA was transcribed with T7 polymerase using a mixture of [alpha-32P]UTP and 4-thio-UTP, yielding a transcript in which approximat...

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Bibliografske podrobnosti
Main Authors: Dontsova, O, Tishkov, V, Dokudovskaya, S, Bogdanov, A, Döring, T, Rinke-Appel, J, Thamm, S, Greuer, B, Brimacombe, R
Format: Artigo
Jezik:Inglês
Izdano: 1994
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC43737/
https://ncbi.nlm.nih.gov/pubmed/7514294
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