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Development of a Molecularly Evolved, Highly Sensitive CaMKII FRET Sensor with Improved Expression Pattern

Genetically encoded fluorescence resonance energy transfer (FRET) biosensors have been successfully used to visualize protein activity in living cells. The sensitivity and accuracy of FRET measurements directly depend on biosensor folding efficiency, expression pattern, sensitivity, and dynamic rang...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Shibata, Akihiro C. E., Maebashi, Hiroshi K., Nakahata, Yoshihisa, Nabekura, Junichi, Murakoshi, Hideji
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4370617/
https://ncbi.nlm.nih.gov/pubmed/25799407
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0121109
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