Rapid and Liquid-Based Selection of Genetic Switches Using Nucleoside Kinase Fused with Aminoglycoside Phosphotransferase

The evolutionary design of genetic switches and circuits requires iterative rounds of positive (ON-) and negative (OFF-) selection. We previously reported a rapid OFF selection system based on the kinase activity of herpes simplex virus thymidine kinase (hsvTK) on the artificial mutator nucleoside d...

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發表在:PLoS One
Main Authors: Tominaga, Masahiro, Ike, Kohei, Kawai-Noma, Shigeko, Saito, Kyoichi, Umeno, Daisuke
格式: Artigo
語言:Inglês
出版: Public Library of Science 2015
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4366196/
https://ncbi.nlm.nih.gov/pubmed/25790096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0120243
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