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Liquid-Based Iterative Recombineering Method Tolerant to Counter-Selection Escapes

Selection-based recombineering is a flexible and proven technology to precisely modify bacterial genomes at single base resolution. It consists of two steps of homologous recombination followed by selection/counter-selection. However, the shortage of efficient counter-selectable markers limits the t...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Tominaga, Masahiro, Kawai-Noma, Shigeko, Kawagishi, Ikuro, Sowa, Yoshiyuki, Saito, Kyoichi, Umeno, Daisuke
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4361647/
https://ncbi.nlm.nih.gov/pubmed/25775434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0119818
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