Mycobacterial DNA Extraction for Whole-Genome Sequencing from Early Positive Liquid (MGIT) Cultures

We developed a low-cost and reliable method of DNA extraction from as little as 1 ml of early positive mycobacterial growth indicator tube (MGIT) cultures that is suitable for whole-genome sequencing to identify mycobacterial species and predict antibiotic resistance in clinical samples. The DNA ext...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Votintseva, Antonina A., Pankhurst, Louise J., Anson, Luke W., Morgan, Marcus R., Gascoyne-Binzi, Deborah, Walker, Timothy M., Quan, T. Phuong, Wyllie, David H., Del Ojo Elias, Carlos, Wilcox, Mark, Walker, A. Sarah, Peto, Tim E. A., Crook, Derrick W.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2015
Assuntos:
Mycobacteriology and Aerobic Actinomycetes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4365189/
https://ncbi.nlm.nih.gov/pubmed/25631807
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.03073-14
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