Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Expression in a Typical Fed-Batch Culture of Pichia pastoris

RESULTS: We have followed a typical fed-batch induction regime for heterologous protein production under the control of the AOX1 promoter using both microarray and metabolomic analysis. The genetic constructs involved 1 and 3 copies of the TRY1 gene, encoding human trypsinogen. In small-scale labora...

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Foilsithe in:PLoS One
Main Authors: Edwards-Jones, Bryn, Aw, Rochelle, Barton, Geraint R., Tredwell, Gregory D., Bundy, Jacob G., Leak, David J.
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2015
Ábhair:
Research Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4364781/
https://ncbi.nlm.nih.gov/pubmed/25785713
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0119637
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