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Heterologous Expression and Efficient Secretion of Chitosanase from Microbacterium sp. in Escherichia coli

A recombinant expression vector, pCT7-CHISP6H, was constructed for the secretory expression of mature peptide of chitosanase (mMschito) from Microbacterium sp. OU01. The vector contains several elements, including T7 promoter, signal peptide sequence of mschito, 6 × His-tag sequence and PmaCI restri...

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Detalhes bibliográficos
Publicado no:Indian J Microbiol
Main Authors: Sun, Yuying, Zhang, Jiquan, Wang, Shujun
Formato: Artigo
Idioma:Inglês
Publicado em: Springer India 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4363261/
https://ncbi.nlm.nih.gov/pubmed/25805906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s12088-014-0505-5
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