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Enhancement of the Efficiency of Secretion of Heterologous Lipase in Escherichia coli by Directed Evolution of the ABC Transporter System

The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased...

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Main Authors: Eom, Gyeong Tae, Song, Jae Kwang, Ahn, Jung Hoon, Seo, Yeon Soo, Rhee, Joon Shick
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology 2005
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1169004/
https://ncbi.nlm.nih.gov/pubmed/16000750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.7.3468-3474.2005
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