Enhancement of the Efficiency of Secretion of Heterologous Lipase in Escherichia coli by Directed Evolution of the ABC Transporter System

The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased...

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Bibliographische Detailangaben
Hauptverfasser: Eom, Gyeong Tae, Song, Jae Kwang, Ahn, Jung Hoon, Seo, Yeon Soo, Rhee, Joon Shick
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2005
Schlagworte:
Physiology and Biotechnology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1169004/
https://ncbi.nlm.nih.gov/pubmed/16000750
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.7.3468-3474.2005
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