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Optimal Cloning of PCR Fragments by Homologous Recombination in Escherichia coli
PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown to most molecular biologists. To popularize t...
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| Publicado no: | PLoS One |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4361335/ https://ncbi.nlm.nih.gov/pubmed/25774528 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0119221 |
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