SMART amplification combined with cDNA size fractionation in order to obtain large full-length clones

BACKGROUND: cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones. Conventionally-generated cDNA libraries contain a high percentage of 5'-truncated clones. Current library construction methods that enrich for full-length mRNA ar...

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Bibliographic Details
Main Authors: Wellenreuther, Ruth, Schupp, Ingo, Poustka, Annemarie, Wiemann, Stefan
Format: Artigo
Language:Inglês
Published: BioMed Central 2004
Subjects:
Methodology Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC436056/
https://ncbi.nlm.nih.gov/pubmed/15198809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2164-5-36
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